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Silva,Gisele Rodrigues da; Fialho,Sílvia Ligório; Siqueira,Rubens Camargo; Jorge,Rodrigo; Cunha Júnior,Armando da Silva. |
The treatment of diseases affecting the posterior segment of the eye is limited by the difficulty in transporting effective doses of drugs to the vitreous, retina, and choroid. Topically applied drugs are poorly absorbed due to the low permeability of the external ocular tissues and tearing. The blood-retina barrier limits drug diffusion from the systemic blood to the posterior segment, thus high doses of drug are needed to maintain therapeutic levels. In addition, systemic side effects are common. Intraocular injections could be an alternative, but the fast flowing blood supply in this region, associated with rapid clearance rates, causes drug concentration to quickly fall below therapeutic levels. To obtain therapeutic levels over longer time periods,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Implants/biodegradation; Drugs/delivery systems; Drugs/prolonged delivery systems; Pharmaceutical technology. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300024 |
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Camilo,Lilian Muniz; Pereira-Chioccola,Vera Lucia; Gava,Ricardo; Meira-Strejevitch,Cristina da Silva; Vidal,Jose Ernesto; Mattos,Cinara Cássia Brandão de; Frederico,Fábio Batista; De Mattos,Luiz Carlos; Spegiorin,Lígia Cosentino Junqueira Franco; Murata,Fernando Henrique Antunes; Ferreira,Marina Neves; Barbosa,Deusenia Machado Ulisses; Gonçalves,Fausto da Silva; Dias,Cristiane Moraes; Catelan,Marcia Wakai; Siqueira,Rubens Camargo; Previato,Mariana; Barbosa,Amanda Pires; Cavallini,Danilo. |
ABSTRACT Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA. The methodology was assayed in 807 clinical samples with known clinical diagnosis, ELISA, and conventional PCR results in a 9-year period. All samples were from patients with clinical suspicion of several features of toxoplasmosis. According to the minimum detection limit curve (in CT), REP-529 had greater sensitivity to detect T. gondii DNA than B1. Both primer sets were retrospectively evaluated using 515... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Symptomatic toxoplasmosis; Diagnosis; Real-time polymerase chain reaction. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600638 |
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